User:Anugraha Raman/Notebook/iGEM 2010/2010/06/23

Project name

Main project page Previous entry      Next entry

Wednesday June 23, 2010 amiRNA Design amiRNAs using online tool Scheme for PCR amiRNA pcr diagram Go to the [amiRNA section] for our primers for this multi step PCR or see chart below: Description Forward (A) Reverse (B) biobrick RS300 CCTTGAATTCGCGGCCGCATCTAGA CCCACAAACACACGCTCGGA AAGGCTGCAGCGGCCGCTACTAGT CCCCATGGCGATGCCTTAAA Description Primer 1 (mir sense) Primer 2 (mir antisense) Primer 3 (mir* sense) Primer 4 (mir* antisense) GFP gaTTAGTGGTCGGCGAGCTGCACtctctcttttgtattcc gaGTGCAGCTCGCCGACCACTAAtcaaagagaatcaatga gaGTACAGCTCGCCGTCCACTATtcacaggtcgtgatatg gaATAGTGGACGGCGAGCTGTACtctacatatatattcct LTP 1 gaTCTAACTATGTATAGGACCACtctctcttttgtattcc gaGTGGTCCTATACATAGTTAGAtcaaagagaatcaatga gaGTAGTCCTATACAAAGTTAGTtcacaggtcgtgatatg gaACTAACTTTGTATAGGACTACtctacatatatattcct Bet v1 gaTTGGTTATATATATCGCACCTtctctcttttgtattcc gaAGGTGCGATATATATAACCAAtcaaagagaatcaatga gaAGATGCGATATATTTAACCATtcacaggtcgtgatatg gaATGGTTAAATATATCGCATCTtctacatatatattcct Transformations Transformed ligations (V0210+LTP1) (V0210+Ger 3 sense) (V0210+Ger 3 antisense)w/turbo cells Grew up colonies w/ RS300 for minipreps