Procedure
- Centrifuged falcon tubes containing gold fibers.
- Removed water with pipette.
- Prepare the sample that goes in the 3mL cuvette that will be used for ocean optics.
- The sample must contain:
- Gold fibers
- (2.996mL) of Tris buffer
- (0.003994mL) of trypsin, calculations are below
- The trypsin was added last, at the second reading such that second reading becomes time 0.
- We ran our sample overnight.
- Readings were taken every 20 minutes overnight. The data was uploaded directly to DropBox for later analysis.
Calculations for volume of trypsin, and volume of buffer
C1·V1 = C2·V2,
where (C1 = 50.21µM)
C2 = 1µM
V2 = 1000µL
(V1 = 0.0199mL)
C1·V1 = C2·V2,
where (C1 = 1µM)
C2 = 0.01µM
V2 = 3000µL
(V1 = 30µL)
Data
Results
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