Task
- Make dilution of HCL stock solution and Glycine stock solution (made last class)
- Record conductivity and pH solutions
- Make a pH and conductivity curve
- Prepare and run SDS-PAGE Running-Buffer
- Analyze dialyzed solutions from yesterday
Dilutions
- Made 8 dilutions in 14 mL Falcon tubes using the volumes of stock solution and water. For each stock solution
- Diluted ×2, 5, 10, 20, 50, 100, 200, 500.
| Dilutions Nacl
|
molarity (mM)
|
pH
|
conductivity (μS/cm)
|
| 2 |
25 |
6.064 |
3030
|
| 5 |
10 |
5.963 |
1178
|
| 10 |
5 |
6.189 |
623
|
| 20 |
2.5 |
5.989 |
304
|
| 50 |
1 |
6.188 |
290.2
|
| 100 |
0.5 |
6.507 |
68
|
| 200 |
0.25 |
6.331 |
75.4
|
| 500 |
0.1 |
6.768 |
50.8
|
| Glycine dilution
|
Molarity (mM)
|
pH
|
Conductivity (μS/cm)
|
| 2 |
25 |
3.479 |
383
|
| 5 |
10 |
3.6 |
201.1
|
| 10 |
5 |
3.704 |
130.5
|
| 20 |
2.5 |
3.835 |
93.9
|
| 50 |
1 |
4.282 |
43.8
|
| 100 |
0.5 |
4.336 |
35.6
|
| 200 |
0.25 |
4.762 |
25.98
|
| 500 |
0.1 |
5.381 |
86
|
|
Project name
Main project page
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