Tasks
- Measure the mean residue liquid in the fiber sample
- 115 μL, 175 μL and 340 μL for a mean of 210 μL
- Measure the absorbance of the different protease concentrations used
- 250 μL samples of protease solutions in Tris/CaCl2 buffer at 1 nM, 10 nM, 100 nM, and 1 μM
- Add 200 mL of 1:4 (Bradford:Buffer) Bradford + 550 mL of additional Tris/CaCl2 b
- 10 nM Chymotrypsin kinetics
- 104.1 µL of the dilute chymotrypsin stock was diluted + 2.896 mL of buffer --> 10 nM
- OceanOptics (used same protocol as previous kinetics runs)
Results
- Protease Bradford results
- AuNP Protease Corrected Bradford Spectra
Note
There was no visible colorimetric change in the solution OceanOptics experiment. Fibers were still present at the end of the allotted reaction time.
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