11/4/12
> Digests (Fermentas FD)
BD-112 and BD-113
Plasmid DNA |
2.0 μl*
|
EcoR1 |
1.0 μl
|
Pst1 |
1.0 μl
|
10x FastDigest buffer + green loading dye |
1.5 μl
|
dH2O |
9.5 μl
|
|
15.0 μl total
|
Incubate at 37°C for 10 minutes.
|
- Make the (1%) agarose glee and add 15μl of restricted DNA (PcTF & RBS) in one well and 10 μl of ladder.
- The size of the constructed gene supposed to be: 1123 + 15 + 6 + 6 + 46 = 1144 bp'
|