09/06/2013
Confirm The Mutagenesis
Plasmid Type |
SD-BD0021 |
SD-BD002 |
SD-BD002 |
BD002 (-) Ctrl |
BD002 (+)Ctrl |
BD002 (+) Ctrl
|
Plasmid DNA |
3.0 μl |
3.0 μl |
3.0 μl |
3.0 μl |
3.0 μl |
3.0 μl
|
BsmBI |
1.0 μl |
0.0 μl |
0.0 μl |
1.0 μl |
0.0 μl |
0.0 μl
|
Spe1 |
0.0 μl |
1.0 μl |
0.0 μl |
0.0 μl |
1.0 μl |
0.0 μl
|
10x FastDigest buffer + green loading dye |
1.5 μl |
1.5 μl |
1.5 μl |
1.5 μl |
1.5 μl |
1.5 μl
|
dH2O |
9.5 μl |
9.5 μl |
10.5 μl |
9.5 μl |
9.5 μl |
10.5 μl
|
|
15.0 μl total |
15.0 μl total |
15.0 μl total |
15.0 μl total |
15.0 μl total |
15.0 μl total
|
Incubate at 37°C for 10 minutes.
|
- Make the (1%) agarose glee and add 15μl of restricted vector in one well and 10 μl of ladder.
- The gel picture shows that the BSMBI cut site did not remove.
|