User:Benjamin Friedel/Notebook/472 CHEM/2016/02/02

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Objective

Todays objective is to prepare gold and silver nanoparticles using pure casein and soy protein isolate as capping and reducing agents

Materials

Stock Solutions

  1. SPI (ARCON S 066400)
  1. Casein (amresco E666-500G)
    1. note store in refrigerator
  1. Fructose
  1. Choloroauric Acid (REM Chemicals)
    1. note store in desiccator, sensitive to metals
  1. Silver Nitrate (CMI 7985)
    1. note store in desiccator, sensitive to light

Procedure

The reaction spreadsheet is shown below. After creating the reaction mixes, samples were heated at 80˚C for 4 hours in the HERATHERM oven in Dr. Harting's Lab in Beeghly (on AU campus). Aluminum foil was used to cover the tubes. This reaction cycle also consisted of 30 minutes of heating and cooling beforehand. Samples were retrieved the next day (1/27/16), checked for color change and then covered in tin foil completely (to prevent oxidation by light of potentially unreacted silver nitrate).

1mL reactions ' ' ' ' ' ' ' ' ' Whey 2.5mM HAuCl4 (µL) 2.5mMAgNO3 (µL) Protein (µL) 2.5mM Fructose (µL) Water (µL) Notes
2.5mM HAuCl4 (µL)2.5mMAgNO3 (µL)Protein (µL)2.5mM Fructose (µL)Water (µL)Au/Ag:ProteinNotes23A400560040023A white fibers
140056004001:1524A400440052024A white fibers
240044005201:1125A400360060025A purple nanoparticles with dark pellet at bottom
340036006001:0926A400280068026A light purple (white and purple) nanofibers at bottom
440028006801:0727A400200076027A slightly pink solution nanoparticles
540020007601:0528A040560060028-33 clear
604056004001:1529A04056020580
704036006001:0930A04056040560
804020007601:0731A0403600600
940056004001:15Formed white fibrous bundles 9-11 (precipitate?)32A04036020580
1040044005201:1133A04036040560
1140036006001:0934A040200060034A, cloudy white fibers at bottom of tube
1240028006801:07purple nanofibers35A04020020580clear
1340020007601:05purple nanofibers36A04020040560clear
1404056006001:1514-22slighty cloudy, white accumulation at bottom (protein precipitate?)
15040560205801:15
16040560405601:15
1704036006001:09
18040360205801:09
19040360405601:09
2004020006001:07
21040200205801:07
22040200405601:07
2340056004001:15*All whey samples have double volume of protein
2440044005201:1123 (white precipitate at bottom)
2540036006001:09(24-27) white stringy fibers climbing tube walls
2640028006801:07
2740020007601:05
2804056006001:1528-30 clear
29040560205801:15
30040560405601:15
3104036006001:0931-33 clear
32040360205801:09
33040360405601:09
3404020006001:0734-36 clear
35040200205801:07
36040200405601:07
3740056004001:1537-40 purple and white fibers
3840044005201:11
3940036006001:09
4040028006801:07
4140020007601:0541 only purple fibers
4204056006001:1542-44 white precipitate at bottom
43040560205801:15
44040560405601:15
4504036006001:0945-47 white precipitate at bottom
46040360205801:09
47040360405601:09
4804020006001:0748-50 white preciptate at bottom
49040200205801:07
50040200405601:07
5140056004001:1551-55 white precipitate with purple fibers near bottom of tube
5240044005201:11


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