User:Bohao Liu/Notebook/Research/2012/10/20

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  • Materials
    • LFM
    • LFM+1M sorbitol
    • Flow Cells
    • Ethanol (70%)
    • H20
    • conA
    • Run fluorescene first as a control or do you think we are ok ???
  • Methods (general)
    • Inoculate cells t = -38 hrs
    • Set back cells t = -14 hrs
    • Set up flow cell
      • plasma clean, holes, etc.
      • wash flow cell with Eth and water
      • add ConA for at least 5 mins
      • measure OD of cells
      • add cells and allow to sit for 5 mins
    • Measurement Parameters
      • Times
        • 210
        • 180
        • 150
        • 120
        • 100
        • 90
        • 80
        • 60
        • 30
        • 10
  • Methods (specific)
    • Set1: 210 and 180
    • Set2: 150 and 120
    • run Set1Wt, Set1Mutant, Set2Wt, Set2 Mutant on one day
    • Set3: 100 and 90 and 80
    • Set4: 60 and 30 and 10
    • run Set3Wt, Set3Mutant, Set4Wt, Set4Mutant on another day
    • Never sleep
    • 5 oscillations per time ie for 60 secs run 11 minutes, start and end with off
    • Wait 20 minutes to allow cells to reset in between different times of the same set
    • I need to go through the timing, but i think i need 3 days to get through each mutant


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