User:Brady P. Dennison/Notebook/Notebook/2015/09/09

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Next entry

Mini prep of KAH60 and KAH128

Decided to use ApaL1 and Xbal for restriction enzymes.

Expected Cuts:
KAH60
288 Base Pairs
1243 Base Pairs
1487 Base Pairs
3769 Base Pairs

KAH128
1934 Base Pairs
4505 Base Pairs

Digest (15 minutes -> 37 degrees):
DNA - 3 microliter
10X Buffer - 3.0 microliter
ApaL1 - microliter
Xbal - 1 microliter
Water - 20 microliter

Gel Image:



Personal tools