User:Brian P. Josey/Notebook/2009/08/28

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How to Make a Gel Anthony showed me how to make a gel today, at least the steps that we could fit in before I leave for class. The steps are: Take either the TAE or TBE buffer, which is already at a 10% concentration in water and make a 50 mL 1% solution. (10mL of the buffer plus 40mL of water) Add 1%, 0.5g, of agrose to the solution and weigh this solution. Heat in the microwave, with cap loose, for a minute or until the agrose completely dissolves. Replace missing water. Pour into the mold with the purple mold piece, missed the name, and let it sit until it hardens, which takes about 30 minutes to an hour. I had to leave for class after these steps, so I will come back and fill the rest of the steps when I get a chance. Note: the glass will be hot when you get it out of the microwave, so use the glove to hold it and pour it over.