# User:Carly M. Montanero/Notebook/CHEM-571/2013/09/11

### From OpenWetWare

< User:Carly M. Montanero | Notebook | CHEM-571 | 2013 | 09

Biomaterials Design Lab: Fall 2013 | Main project page Previous entry Next entry |

## Objective- To finish the procedure from yesterday.
- Use the Atomic Absorption Spectrometer to test gold standards, citrate-AuNP solutions and BSA-AuNP solutions.
## Procedure- There was an error in placing the cuvette in the UV-Vis, so all of our data from yesterday is incorrect. We created new standards and reran each sample, correctly using the UV-Vis.
- Today, we measured the concentrations in 10ml volumetric flask and the cuvette had a path length of 3cm.
- Standard solutions of gold and previously made standards of citrate-AuNP and BSA-AuNP were analyzed with the Atomic Absorption Spectrometer.
## Figures
- Atomic Absorption Data for Gold Standard, BSA-AuNP Standard, and Citrate-AuNP Standard
## Calculating the Number of Gold Atoms in Citrate-AuNP- The equation of the best fit line from the class data was y = 3x10
^{-5}x^{2}+ 0.0209x. - The average of our corrected citrate-AuNP concentration was 0.1444 μg/mL.
- Setting 0.1444 equal to y yields the equation 0.1444 = 3x10
^{-5}x^{2}+ 0.0209x. - Solving the quadratic equation gives two answers: x = -703.51 and x = 6.842.
- Disregarding the negative value, the 6.842 μg/mL corresponds to the concentration of gold.
- We diluted the sample of gold by 10, so the actual concentration is 0.06842 g/L.
- The molecular weight of gold is 196.97 g/mol.
- Converting the concentration of gold to molarity yields a concentration of 3.474x10
^{-4}M gold. - Dividing the concentration of gold (3.474x10
^{-4}) M by the concentration of citrate-AuNP ( 9.17×10^{-9}M) gives the final number of gold atoms per citrate-AuNP molecule. - Though these calculations, there are 37,884 gold atoms per citrate-AuNP molecule.
## NotesThe UV-Vis spectra of trial 4 Bradford Assay of horseradish peroxidase had some serious errors. We discarded the data. |