User:Carly M. Montanero/Notebook/CHEM-571/2013/10/29

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Biomaterials Design Lab: Fall 2013 Main project page
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Objective

Analysis and synthesis of more protein-AuNP materials. Each group will have a different task. One (set) of groups will be doing AA on their samples from last weed (HRP-AuNPs and pepsin-AuNPs) and this week (Myoglobin-AuNPs and HRP-AuNPs). Another (set) will take UV-Vis absorbance of their samples (this week and last week). The final (set) will synthesize new protein-AuNPs.

Procedure

Creating the Gold Stock Solution

  1. Add 0.0506 g of HAuCl4·3H2O to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of gold stock was 2.57 mM.

Creating the Lysozyme Stock Solution

  1. Add 0.010725 g of lysozyme to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of lysozyme stock was 15 μM.

Creating the Myoglobin Stock Solution

  1. Add 0.02640 g of myoglobin to a 50 mL volumetric flask and fill with distilled water.
  2. The final concentration of myoglobin stock was 15 μM.

Synthesizing Lysozyme AuNP and Myoglobin AuNP

Solutions with the following Au:protein ratio were synthesized:

Gold to Lysozyme Ratio
  • 30
  • 50
  • 70
  • 90
  • 110
  • 130
  • 150
  • 170
  • 190
  • 210
Gold to Myoglobin Ratio
  • 10
  • 15
  • 25
  • 30
  • 35
  • 40
  • 45
  • 50
  1. As seen in Figure 1, the corresponding amount of protein was added to a 50 mL volumetric flask and filled with distilled water. The samples were made in small test tubes and capped with aluminum foil.
  2. The samples were heated at 80°C for 4 hours.

Figures

Image:10.29.2013_cmj_solutions1.png Image:10.29.2013_cmj_solutions2.png


  • Myoglobin UV-Vis

Image:11.5.2013_cmj_UVVis_myo.png


  • Lysozyme UV-Vis

Image:11.5.2013_cmj_UVVis_lyzo.png

Notes

  • The final gold concentration in the solutions was 0.25 mM.



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