User:Cassandra M Barrett/Notebook/Haynes Lab/2015/08/21

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T2A-EGFP Phusion PCR Insert content here... Purpose: To amplify T2A-EGFP fragment from pX330g plasmid for insertion into new vector Materials: Primers P231/P232 pX330g Phusion Reagents Methods: Tempate was diluted 1:1000 Set up reaction as follows Water 32.5uL HFmm 10uL dNTPs 1uL Forward Primer 2.5uL Reverse Primer 2.5uL Template 1uL Phusion Enzyme 0.5uL Run on Phusion PCR program at 60C Results: Gel revealed failed PCR, reattempt