User:Cassidy B. Hart/Notebook/CHEM-471: Gold & Silver Nanoparticles/2016/11/02

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Ocean Optics

Procedure

  1. Sample was made Myoglobin: 0.25mM Au, 12.5uM Myoglobin, 3mL total
    1. Au 3.67mM
      1. (0.25mM)(3.0mL)=(XmL)(3.67mM)
      2. XmL= .204mL= 204uL Au
    2. Myoglobin 103uM
      1. (12.5uM)(3.0mL)=(XmL)(103uM)
      2. X=0.364mL= 364uL Myoglobin
    3. 30uL 1mM NaOH
    4. Water
      1. Volume of water= 3000uL- 204uL-364uL-30uL= 2402uL
    5. Scans were taken at 80oC every 2 minutes for 3 hours

Data

Absorbance


Wavelength


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