- To find the sensitivity of the fluorometer using Ru(bpy)(phen) in acetonitrile.
First, standards of Ru(bpy)(phen) in acetonitrile were made. The stock solution of Ru(bpy)(phen) is 360μM:
|Standard ||Amount of Ru(bpy)(phen) ||Amount of acetonitrile ||Final Concentration in Cuvette
||60 μL ||2940 μL ||7.2 μM
||120 μL ||2880 μL ||14.4μM
||180μL ||2820μL ||21.6μM
||240μL ||2760μL ||28.8μM
||300μL ||2700μL ||36μM
||600μL ||2400μL ||72μM
||900μL ||2100μL ||108μM
The intensities taken were corrected with the intensities of acetonitrile, and the spectrum of intensities was graphed:
The curves were integrated in Excel and the following areas under the curves were found:
|Concentration (μM) ||Integrated Intensity
These integrated intensity were plotted against the concentrations to see the sensitivity of the instrument:
The calculated integrated intensities for all data collected from the class was graphed in the same way:
This area is for any observations or conclusions that you would like to note.
Use categories like tags. Change the "Course" category to the one corresponding to your course. The "Miscellaneous" tag can be used for particular experiments, as instructed by your professor. Please be sure to change or delete this tag as required so that the categories remain well organized.