User:Charlynn Burgess/Notebook/ATP Synthesis/2011/12/01

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Solubility Test

  1. Place small amount of solid ATP-Ru in 5 different solvents: MeOH, CH3CN, EtOH, DMF, and Distilled H2O
  2. Let sit for ~ 10 min

Observations:

  1. ATP- Ru was soluble only in the distilled H2O


Method #2

  1. Soaked filter paper with solid ATP-Ru in distiller water for about 30 min
  2. Filtered ATP-Ru distilled water solution
  3. Ran solution on TLC using 50:50 CH3CN/H2O as solvent


Matt Hartings UV Vis and Fluorescence data were collected later in the day from this workup A small portion was randomly drawn from the stock and diluted to make all of the samples Samples were made in 2 cuvettes. Cuvette 1 is a quartz cuvette with frosted sides (1cm path length and holds around 1mL of sample). Cuvette 2 is a quartz cuvette with 4 quartz sides for use in a fluorometer.

For the UV Vis graphs: The two graphs show a different scaling for the data in order to show the absorbance features of each sample. Cuvettes 1 and 2 were used in this data as indicated. Each data set is corrected to remove the absorbance contributions of the solvent (water).



For the fluorescence graphs: For each sample a 450nm excitation beam was used and the fluorescence was recorded from 500nm to 700nm. Each spectrum has been corrected to remove the contributions of the solvent (water). The two graphs are separated due to different experimental conditions. Graph 1 used 10nm excitation and emission slits and ran at a speed of 500nm/min. Graph 2 used 5nm excitation and emission slits and ran at a speed of 60nm/min. The names of the spectra shown here correspond to the UV vis spectra taken in the fluorescence cuvette as shown in the figures above.





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