User:Cheuk Ka Tong/Sandbox4

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Contents

Modelling

In-Vitro Expression

ATP regenerating systems

  • ADP + phosphoenolpyruvate \longrightarrow ATP + pyruvate

Pyruvate kinase (PK) catalyses the final step in glycolysis, the conversion of phosphoenolpyruvate to pyruvate with concomitant phosphorylation of ADP to ATP. The enzyme requires both magnesium and potassium ions for its activity.

  • ADP + creatine phosphate \longrightarrow ATP + creatine

Creatine kinase (CK) catalyses the conversion of creatine phosphate to creatine with concomitant phosphorylation of ADP to ATP. This enzyme acts in tissues that consume ATP rapidly, especially in skeletal muscle.

ATP expenditure

  • Per amino acid incorporated

Energy efficiency of the expression reaction

Vesicle Formation

  1. Efficiency of encapsulation in bilayer vesicles
    • Emulsification of vesicle content
    • Transfer from lipid-oil to aqueous phase
  2. Probability of gene expression
    • Size of vesicles vs. size of E. coli
    • Density of vesicles
    • Lifespan of vesicles
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