User:Chris Stubbs

Contact Info

Protein Kinase C C1-domain interaction with membrane

• Chris Stubbs
• Central Laser Facility, LSF R25a, Rutherford Appleton Laboratory,Chilton, Didcot, OX11 0QXUK
• Email me through OpenWetWare or (pref) cdstubbs@sent.com

Education

• PhD, U. London

Research interests

1. Protein kinase C, C1-domain-ligand interactions, PKC signalling
2. Fluorescence lifetime imaging spectroscopy
3. Cell membranes

Publications (selected)

1. Stubbs CD, Botchway SW, Slater SJ, and Parker AW. The use of time-resolved fluorescence imaging in the study of protein kinase C localisation in cells. BMC Cell Biol. 2005 Apr 26;6(1):22. DOI:10.1186/1471-2121-6-22 |
2. Slater SJ, Malinowski SA, and Stubbs CD. The nature of the hydrophobic n-alkanol binding site within the C1 domains of protein kinase Calpha. Biochemistry. 2004 Jun 15;43(23):7601-9. DOI:10.1021/bi049755z |
3. Slater SJ, Cook AC, Seiz JL, Malinowski SA, Stagliano BA, and Stubbs CD. Effects of ethanol on protein kinase C alpha activity induced by association with Rho GTPases. Biochemistry. 2003 Oct 21;42(41):12105-14. DOI:10.1021/bi034860e |
4. Slater SJ, Ho C, and Stubbs CD. The use of fluorescent phorbol esters in studies of protein kinase C-membrane interactions. Chem Phys Lipids. 2002 Jun;116(1-2):75-91. DOI:10.1016/s0009-3084(02)00021-x |
5. Slater SJ, Seiz JL, Cook AC, Buzas CJ, Malinowski SA, Kershner JL, Stagliano BA, and Stubbs CD. Regulation of PKC alpha activity by C1-C2 domain interactions. J Biol Chem. 2002 May 3;277(18):15277-85. DOI:10.1074/jbc.M112207200 |
6. Ho C, Slater SJ, Stagliano B, and Stubbs CD. The C1 domain of protein kinase C as a lipid bilayer surface sensing module. Biochemistry. 2001 Aug 28;40(34):10334-41. DOI:10.1021/bi002839x |
7. Slater SJ, Seiz JL, Stagliano BA, Cook AC, Milano SK, Ho C, and Stubbs CD. Low- and high-affinity phorbol ester and diglyceride interactions with protein kinase C: 1-O-alkyl-2-acyl-sn-glycerol enhances phorbol ester- and diacylglycerol-induced activity but alone does not induce activity. Biochemistry. 2001 May 22;40(20):6085-92. DOI:10.1021/bi001002z |
8. Slater SJ, Stagliano BA, Seiz JL, Curry JP, Milano SK, Gergich KJ, and Stubbs CD. Effects of ethanol on protein kinase C activity induced by filamentous actin. Biochim Biophys Acta. 2001 Jan 12;1544(1-2):207-16. DOI:10.1016/s0167-4838(00)00222-3 |
9. Slater SJ, Seiz JL, Stagliano BA, and Stubbs CD. Interaction of protein kinase C isozymes with Rho GTPases. Biochemistry. 2001 Apr 10;40(14):4437-45. DOI:10.1021/bi001654n |
10. Slater SJ, Milano SK, Stagliano BA, Gergich KJ, Curry JP, Taddeo FJ, and Stubbs CD. Interaction of protein kinase C with filamentous actin: isozyme specificity resulting from divergent phorbol ester and calcium dependencies. Biochemistry. 2000 Jan 18;39(2):271-80. DOI:10.1021/bi9916527 |
11. Ho C, Slater SJ, Stagliano BA, and Stubbs CD. Conformation of the C1 phorbol-ester-binding domain participates in the activating conformational change of protein kinase C. Biochem J. 1999 Dec 1;344 Pt 2(Pt 2):451-60.
12. Stubbs CD and Slater SJ. Ethanol and protein kinase C. Alcohol Clin Exp Res. 1999 Sep;23(9):1552-60.
13. Slater SJ, Taddeo FJ, Mazurek A, Stagliano BA, Milano SK, Kelly MB, Ho C, and Stubbs CD. Inhibition of membrane lipid-independent protein kinase Calpha activity by phorbol esters, diacylglycerols, and bryostatin-1. J Biol Chem. 1998 Sep 4;273(36):23160-8. DOI:10.1074/jbc.273.36.23160 |
14. Ho C and Stubbs CD. Effect of n-alkanols on lipid bilayer hydration. Biochemistry. 1997 Sep 2;36(35):10630-7. DOI:10.1021/bi9703150 |
15. Slater SJ, Kelly MB, Larkin JD, Ho C, Mazurek A, Taddeo FJ, Yeager MD, and Stubbs CD. Interaction of alcohols and anesthetics with protein kinase Calpha. J Biol Chem. 1997 Mar 7;272(10):6167-73. DOI:10.1074/jbc.272.10.6167 |
16. Stubbs CD and Slater SJ. The effects of non-lamellar forming lipids on membrane protein-lipid interactions. Chem Phys Lipids. 1996 Jul 15;81(2):185-95. DOI:10.1016/0009-3084(96)02581-9 |
17. Slater SJ, Kelly MB, Yeager MD, Larkin J, Ho C, and Stubbs CD. Polyunsaturation in cell membranes and lipid bilayers and its effects on membrane proteins. Lipids. 1996 Mar;31 Suppl:S189-92. DOI:10.1007/BF02637074 |
18. Slater SJ, Ho C, Kelly MB, Larkin JD, Taddeo FJ, Yeager MD, and Stubbs CD. Protein kinase Calpha contains two activator binding sites that bind phorbol esters and diacylglycerols with opposite affinities. J Biol Chem. 1996 Mar 1;271(9):4627-31. DOI:10.1074/jbc.271.9.4627 |
19. Ho C, Slater SJ, and Stubbs CD. Hydration and order in lipid bilayers. Biochemistry. 1995 May 9;34(18):6188-95. DOI:10.1021/bi00018a023 |
20. Slater SJ, Kelly MB, Taddeo FJ, Larkin JD, Yeager MD, McLane JA, Ho C, and Stubbs CD. Direct activation of protein kinase C by 1 alpha,25-dihydroxyvitamin D3. J Biol Chem. 1995 Mar 24;270(12):6639-43. DOI:10.1074/jbc.270.12.6639 |
21. Ho C, Kelly MB, and Stubbs CD. The effects of phospholipid unsaturation and alcohol perturbation at the protein/lipid interface probed using fluorophore lifetime heterogeneity. Biochim Biophys Acta. 1994 Aug 3;1193(2):307-15. DOI:10.1016/0005-2736(94)90167-8 |
22. Slater SJ, Kelly MB, Taddeo FJ, Ho C, Rubin E, and Stubbs CD. The modulation of protein kinase C activity by membrane lipid bilayer structure. J Biol Chem. 1994 Feb 18;269(7):4866-71.
23. Ho C, Williams BW, Kelly MB, and Stubbs CD. Chronic ethanol intoxication induces adaptive changes at the membrane protein/lipid interface. Biochim Biophys Acta. 1994 Jan 19;1189(2):135-42. DOI:10.1016/0005-2736(94)90058-2 |
24. Rooney TA, Hager R, Stubbs CD, and Thomas AP. Halothane regulates G-protein-dependent phospholipase C activity in turkey erythrocyte membranes. J Biol Chem. 1993 Jul 25;268(21):15550-6.
25. Slater SJ, Cox KJ, Lombardi JV, Ho C, Kelly MB, Rubin E, and Stubbs CD. Inhibition of protein kinase C by alcohols and anaesthetics. Nature. 1993 Jul 1;364(6432):82-4. DOI:10.1038/364082a0 |
26. Stubbs CD and Rubin R. Effect of ethanol on platelet phospholipase A2. Lipids. 1992 Apr;27(4):255-60. DOI:10.1007/BF02536471 |
27. Ho C, Williams BW, and Stubbs CD. Analysis of cell membrane micro-heterogeneity using the fluorescence lifetime of DPH-type fluorophores. Biochim Biophys Acta. 1992 Mar 2;1104(2):273-82. DOI:10.1016/0005-2736(92)90041-j |
28. Stubbs CD, Kisielewski AE, and Rubin E. Chronic ethanol ingestion modifies liver microsomal phosphatidylserine inducing resistance to hydrolysis by exogenous phospholipase A2. Biochim Biophys Acta. 1991 Dec 9;1070(2):349-54. DOI:10.1016/0005-2736(91)90076-k |
29. Stubbs CD and Smith AD. Essential fatty acids in membrane: physical properties and function. Biochem Soc Trans. 1990 Oct;18(5):779-81. DOI:10.1042/bst0180779 |
30. Stubbs CD and Kisielewski AE. Effect of increasing the level of omega-3 fatty acids on rat skeletal muscle sarcoplasmic reticulum. Lipids. 1990 Sep;25(9):553-8. DOI:10.1007/BF02537164 |
31. Williams BW, Scotto AW, and Stubbs CD. Effect of proteins on fluorophore lifetime heterogeneity in lipid bilayers. Biochemistry. 1990 Apr 3;29(13):3248-55. DOI:10.1021/bi00465a016 |
32. Stubbs CD, Williams BW, Boni LT, Hoek JB, Taraschi TF, and Rubin E. On the use of N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)phosphatidylethanolamine in the study of lipid polymorphism. Biochim Biophys Acta. 1989 Nov 17;986(1):89-96. DOI:10.1016/0005-2736(89)90276-9 |
33. Nie Y, Stubbs CD, Williams BW, and Rubin E. Ethanol causes decreased partitioning into biological membranes without changes in lipid order. Arch Biochem Biophys. 1989 Jan;268(1):349-59. DOI:10.1016/0003-9861(89)90596-1 |
34. Williams BW and Stubbs CD. Properties influencing fluorophore lifetime distributions in lipid bilayers. Biochemistry. 1988 Oct 18;27(21):7994-9. DOI:10.1021/bi00421a004 |
35. Stubbs CD, Williams BW, Pryor CL, and Rubin E. Ethanol-induced modifications to membrane lipid structure: effect on phospholipase A2-membrane interactions. Arch Biochem Biophys. 1988 May 1;262(2):560-73. DOI:10.1016/0003-9861(88)90407-9 |
36. Hoek JB, Harada N, Moehren G, Tomsho M, and Stubbs CD. The role of calcium and phospholipase A2 in glucagon-induced enhancement of mitochondrial calcium retention. Adv Exp Med Biol. 1988;232:25-36. DOI:10.1007/978-1-4757-0007-7_3 |
37. Smith AD and Stubbs CD. Modulation of membrane protein function by bilayer lipids. Basic Res Cardiol. 1987;82 Suppl 1:93-7. DOI:10.1007/978-3-662-08390-1_11 |
38. Stubbs CD, Kinosita K Jr, Munkonge F, Quinn PJ, and Ikegami A. The dynamics of lipid motion in sarcoplasmic reticulum membranes determined by steady-state and time-resolved fluorescence measurements on 1,6-diphenyl-1,3,5-hexatriene and related molecules. Biochim Biophys Acta. 1984 Sep 5;775(3):374-80. DOI:10.1016/0005-2736(84)90193-7 |
39. Stubbs CD and Smith AD. The modification of mammalian membrane polyunsaturated fatty acid composition in relation to membrane fluidity and function. Biochim Biophys Acta. 1984 Jan 27;779(1):89-137. DOI:10.1016/0304-4157(84)90005-4 |
40. Stubbs CD, Kouyama T, Kinosita K Jr, and Ikegami A. Effect of double bonds on the dynamic properties of the hydrocarbon region of lecithin bilayers. Biochemistry. 1981 Jul 21;20(15):4257-62. DOI:10.1021/bi00518a004 |
41. Stubbs CD, Tsang WM, Belin J, Smith AD, and Johnson SM. Incubation of exogenous fatty acids with lymphocytes. Changes in fatty acid composition and effects on the rotational relaxation time of 1,6-diphenyl-1,3,5-hexatriene. Biochemistry. 1980 Jun 10;19(12):2756-62. DOI:10.1021/bi00553a034 |

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