User:Christine Doan/Notebook/Identifying Kinase Substrates of PfCK2/2014/02/18

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Primer Design DC approved Old CK2 DNA stock Andrew made a regular and a digest DNA gel Select two enzymes to digest miniprepped old CK2 DNA from stock Verify size of digests before making dilutions to send off for sequencing 20ul reaction 1ul NdeI 1ul XhoI 2ul Buffer 4 2ul 10x BSA 5ul DNA (concentration >100ng/ul) 9ul dH2O Expected sizes: 1100 and 5370 Mystery Adam's notebook reads: pet29b, BL21 (DE3) A box 7, #34 B box 7, #38 Glycerol stock reads: A 5.31.01 B XLI Blue 5.28.01 DNA stock reads: 395 | CKII pDNA 105ng/ul 1/26/01 Mystery solved: Valerie Bracchi-Ricard cloned it into pET 29b