User:Cosette Brianna Taggart/Notebook/Experimental/2016/09/07

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry

September 7, 2016 : Ocean Optics I

Objective

  1. Run Ocean optics on sample prepped today.

Description

Ocean Optics

  1. Sample was prepped according to Lysozyme prep seen in data
  2. Sample was placed into Ocean Optics Machine
  3. Machine was run for 3 hours collecting data every 2 minutes

Data

Stock solutions

  1. Lysozyme
    1. 8.88 mg of lysozyme with water in 10.0 mL volumetric flask
    2. MW = 14307 g/mol
    3. concentration = 62.1 μM
  2. AuCl3
    1. 38.5 mg of AuCl3 with water in 25.0 mL volumetric flask
    2. MW = 303.3 g/mol
    3. concentration = 5.08 mM

Instructions for making sample for ocean optics

  1. pH 4
    1. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total
      1. 147 μL AuCl3
      2. 300 μL 1M HCl
      3. 245 μL Lysozyme
      4. 2.308 mL Water


Summary Data at 30 minute increments
Time(minutes) Maximum Absorbance Maximum Wavelength(nm)
0 0 449.84
30 0.0477146 570.11
60 0.205807 543.15
90 0.364907 543.15
120 0.446653 535.15
150 0.522327 534.11
178 0.562715 533.76



Personal tools