User:Cosette Brianna Taggart/Notebook/Experimental/2016/09/21

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September 21, 2016 : Fluorescence III

Objective

  1. Collect fluorescence data for lysozyme at pH 6
  2. Make stock solutions for UV-Vis on 9.27.2016

Description

Fluoresence

  1. Lysozyme solution was made according to the measurements found in data below
  2. Lysozyme solution was run through florescence for 3 hours with data taken every 5 minutes

Stock Solutions for 9.27.2016

Solutions were made in glass test tubes, covered with foil, and placed in an oven for 4 hours at 80 C.

Data

Fluorescence

  1. Solution Measurements
    1. Lysosome Stock Solution
      1. concentration = 225 μM
    2. Gold Stock Solution
      1. concentration = 3.73 mM
    3. Lysozyme: 0.25mM Au, 12.5μM Lysozyme, 3mL total; pH 6
      1. 223 μL AuCl3
      2. 3 μL 1M HCl
      3. 167 μL Lysozyme
      4. 2607 μL Water

SUMMARY DATA AND FIGURES

Summary Data at 15 minute increments
Time(minutes) Maximum Emission Integrated Intensity
0 423.5 3712.15643
15 420 7231.99931
30 423 6161.526628
45 427 5814.874805
60 428.5 6106.685527
75 426 6387.85191
90 426 6671.018707
105 430.5 7022.28452
120 427 7297.185504
135 428.5 7504.95303
150 426 7665.567058
165 430 7820.376345
180 425 8021.858662



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