Objective
Make Myoglobin and Lysozyme nano particles
Description
- Lysozyme AuNP and Hemoglobin AuNP
- Determine the total amounts of materials you will need for the following samples using the same procedures as the last two times we made protein-AuNPs (final Au concentration of 0.25mM and a final volume of 5mL)
- Myoglobin
- 5
- 10
- 15
- 20
- 25
- 30
- 35
- 40
- 45
- 50
- Lysozyme
- 30
- 50
- 70
- 90
- 110
- 130
- 150
- 170
- 190
- 210
- Make your stock solutions
- Make your samples in small test tubes and cap with aluminum foil
- Heat your samples at 80C for 4 hours
Data
The stock solutions we made with the following concentrations
- Lysozyme
- 5 mg in 25mL of water ---> 13.98 μM
- Myoglobin
- 60 mg in 25mL of water ---> 70.66 μM
- Gold
- Need 50mL of 2.5mM
- 44.4 mg of HAuCl4·3H2O in 50mL water --> 2.25 mM
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