User:Daniel-Mario Larco/Notebook/AU Biodesign Lab - 09/03/2013/2014/02/12
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Monitoring Activity of HRP-AuNP complexes
UV-Vis spectra were recorded for solutions over a period of time
The formation of 2,3-Diaminophenazine will produce a peak between 350 and 400 nm. The spectra of both solution were compared over time to determine whether or not there were active HRP molecule with the AuNPs. The results showed that the solution containing the HRP-AuNPs had a significantly faster rate of formation for 2,3-Diaminophenazine. This result confirms the presence of active HRP molecule in the AuNP solution. The next step is determining whether or not these active HRP molecules are attaches to the nano particles.