User:Daniel-Mario Larco/Notebook/AU Photosynthesis Lab/2014/06/30

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Procedure

  1. The dialysis tubes with the protein in ph5 Acetate buffer were collected
    1. All the protein crashed out
  2. The solutions were spun down at 20000 rpm for 30 minutes at 4C
    1. The supernatant was clear, supporting the claim that everything crashed out of solution
  3. The pellet was resuspended in 10mM ph10 CHES buffer
    1. Buffer prepared by dissolving .207g of CHES in 100mL of H2O and pH adjusted with 1M NaOH
    2. In order to help dissolving the pellet, the pellet and buffer were placed in a beaker with a stir bar
    3. This beaker was placed in a larger beaker with ice before placing on stir plate
  4. Once dissolved, the solution was filtered
  5. The filtered solution was stored in the fridge overnight



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