User:Daniel A. Vargas/Notebook/General lab notebook/2015/06/29

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PCR amplification of chromatin remodeling

Reagent Volume Expected:
1. ANM5 = 1911,
2. MYB = 159,1764
3. Carm1 = 1827
4. SETD7 = 459,642
5. KMT2E = 1176,4401
6. SETD1A = 420,4704
Image:Http://openwetware.org/images/4/48/Screen Shot 2015-05-14 at 1.14.53 PM.png
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 1.0 μL
1X GoTaq 12.5
F primer 1.0
R Primer 1.0
dH2O 9.5
  25 μL

Transforming P300 plasmids

Transformation protocol

Sample ID Expected Observed colonies
PBS P300GrowthNone0.00
NHA P300GrowthNone0.00
CHA P300GrowthNone0.00

Plasmids were sent on filter paper and is possible did not remain intact during transit.


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