User:Daniel A. Vargas/Notebook/General lab notebook/2015/06/30

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Gel electrophoresis of PCR amp

Reagent Volume Expected:
1. ANM5 = 1911,
2. MYB = 159,1764
3. Carm1 = 1827
4. SETD7 = 459,642
5. KMT2E = 1176,4401
6. SETD1A = 420,4704
Damaged Gel
15 μL/lane; 1% agarose; Ladder
DNA(plasmid) 1.0 μL
1X GoTaq 12.5
F primer 1.0
R Primer 1.0
dH2O 9.5
  25 μL

Program: Gotaq35cyclo (block A lane5)

  • 95°C, 3 min
  • 35x[95°C, 10 sec; 48°C, 30 sec; 72°C, 120 sec]
  • 72°C, 5 min
  • 4°C ∞

Program: Gotaq35cyclo (block B 1-4, and 6)

  • 95°C, 3 min
  • 35x[95°C, 10 sec; 64°C, 30 sec; 72°C, 120 sec]
  • 72°C, 5 min
  • 4°C ∞

Gel fell and was damaged, PCR will need to be redone. May be better to run a restriction enzyme digest in the future for more accurate results.



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