User:Daniel A. Vargas/Notebook/General lab notebook/2015/07/15

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Plasmid Validation Restriction Digest Table Checked plasmid minipreps with EcoRI/Xbal/ApaL1/Bsa1 digests Reagent 1 2 3-5 Volume Expected: 1. ANM5 = 4,261 2. MYB = 4,483 3. Carm1 = 4,418 4. KMT2E = 4,418 5. SETD1A = 7,715 30 μL/lane; 1% agarose; Ladder DNA(plasmid) 3.0 μL 10X buffer 3.0 EcoRI 1 0 1 1.0 Xbal 0 1 1 1.0 ApaL1 1 0 0 1.0 Bsa1 0 1 0 1.0 dH2O 20   30 μL --> 37°C/ 15 min.