User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/08/06

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Wet Lab: OmpC/F promoter Electrophoresis Gel OmpF PCR_RTTH In order to probe the PCRs reactions an electroforesis gel was made. This was loadded as follows: Lane1. Ladder Lane2. PCR1 (pSB3K3 + GFP_OmpF) Lane3. PCR2 (pSB3K3 + GFP_control_bluepromoter) Lane4. PCR3 (pSB3K3 + GFP_control) Lane5. PCR4 (pSB1C3_OmpC) Lane6. PCR5 (pSB1C3_control) The PCR product is between 2000-2500bp It is had PCR product from the pSB1C3 backbone. The next step is to run a second PCR reaction with the other OmpF REV primer, using as template the product from the PCR4. Note [pSB1C3]