Modeling: PoPS
From de article: "Measuring the activity of BioBrick promoters using an in vivo reference standard"
- Promoter activity. Number of RNAp molecules that pass by the final base pair of the promoter and continue along DNA as an elongation complex.
- PoPs (Absolute activity of promoter). Rate of successful mRNA initiation events per DNA copy of each promoter. Number of elongating polymerases per second (PoPs) exiting the promoter.
- RPUs (Relative activity of promoter). Ratio of absolute activity of the promoter to the absolute activity of a reference standard promoter measure under the same conditions and with the same measurement instruments.
BioBrick Plasmids
- [pSB3K3]
- p15A origin of replication
- 10-12 copy number
- kn resistance
- [pSB4T5]
- pSC101 origin of replication
- ~5 copy number
- tet resistance
- [pSB3C5]
- p15A origin of replication
- unknown copy number
- cm resistance
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