User:Daniela A. Garcia S./Notebook/Modeling UNAM-Genomics Mexico/2010/08/25

Project name

Main project page Previous entry      Next entry

WetLab: LovTap + promoters Transformation report The transformation from 240810 got well, for all the promoters a considerable amount of colonies were gotten. Transformation The ligations from 240810 were transformed into DH5α competent cells following the next protocol. These have the LovTap product from the colony number_9. 20' on ice 1' @ 42ºC 5' on ice 1h 1mL of LB 1' @ 3000rmp As a positive control was used the plasmid N.19[Am]