User:Dave Casey/Notebook/OpenQPCR

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Notes

Started around 8:30pm added:

  • 80uL of 2X chai master
  • 24uL ITFS primer mix
  • 32uL distilled water

mix, alloquot 17uL into 8 0.2mL PCR tubes

set up openQPCR with he following program:

  • 3:00 95C

then 40X:

  • 0:30 95C
  • 0:30 55C
  • 0:30 72C

Then melt curve.

Meanwhile, we set up a pi so we can take these notes on the big screen

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