User:David Benjamin Nyer/Notebook/PcTF breast cancer/2015/11/03

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Summary

Here are the neon transfection results:

There's a pretty clear correlation between increasing voltage and both increased transfection efficiency AND decreased cell recovery. Trick will be to find the sweet spot between the two extremes...


BT-474 data:

Sample Well no. Pulse voltage Pulse width Pulse no. Transfection Efficiency Live (cells/µL) Dead (cells/µL) Cell Viability Cell Viability (normalized to control)
1A1---0.10%420598.82%100.00%
2A2140020131.91%33782.50%7.86%
3A3150020152.07%121250.00%2.86%
4A4160020167.65%3827.27%0.71%
5A5170020174.42%91636.00%2.14%
6A6110030127.72%951090.48%22.62%
7B1120030139.80%49885.96%11.67%
8B2130030143.37%8657.14%1.90%
9B3140030171.02%18772.00%4.29%
10B4100040129.46%114794.21%27.14%
11B5110040142.74%36783.72%8.57%
12B6120040168.75%431672.88%10.24%
13C1110020241.21%81792.05%19.29%
14C2120020258.26%73989.02%17.38%
15C3130020277.55%20483.33%4.76%
16C4140020287.93%12475.00%2.86%
17C585030218.07%1092283.21%25.95%
18C695030242.12%1622984.82%38.57%
19D1105030258.86%1381490.79%32.86%
20D2115030271.43%4266.67%0.95%
21D3130010343.27%1275569.78%30.24%
22D4140010353.64%341668.00%8.10%
23D5150010368.13%521281.25%12.38%
24D6160010375.87%321469.57%7.62%

BT-549 data:

Sample Well no. Pulse voltage Pulse width Pulse no. Transfection Efficiency Live (cells/µL) Dead (cells/µL) Cell Viability Cell Viability (normalized to control)
1A1---0.24%1632586.70%100.00%
2A2140020135.08%50590.91%30.67%
3A3150020136.76%19386.36%11.66%
4A4160020143.33%18290.00%11.04%
5A5170020156.67%3175.00%1.84%
6*A611003010.48%1244573.37%76.07%
7B1120030129.48%50984.75%30.67%
8B2130030137.29%24582.76%14.72%
9B3140030133.33%2166.67%1.23%
10B4100040119.64%621184.93%38.04%
11B5110040130.50%34587.18%20.86%
12B6120040141.74%23388.46%14.11%
13C1110020223.41%551677.46%33.74%
14C2120020231.05%441081.48%26.99%
15C3130020242.46%18385.71%11.04%
16C4140020244.58%8280.00%4.91%
17C585030212.60%892578.07%54.60%
18C695030226.27%571282.61%34.97%
19D1105030230.96%24777.42%14.72%
20*D211503020.64%943871.21%57.67%
21D3130010322.56%39784.78%23.93%
22D4140010329.59%27681.82%16.56%
23D5150010337.84%11568.75%6.75%
24D6160010350.65%8280.00%4.91%



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