Summary
Ideal neon transfection conditions for BT-474 and BT-549 cell lines (so far):
BT-474
| Pulse voltage
|
Pulse width
|
Pulse no.
|
Transfection Efficiency
|
Cell Recovery (normalized to control)
|
| 1050 |
30 |
2 |
58.86% |
32.86%
|
| 1300 |
10 |
3 |
43.27% |
30.24%
|
| 950 |
30 |
2 |
42.12% |
38.57%
|
BT-549
| Pulse voltage
|
Pulse width
|
Pulse no.
|
Transfection Efficiency
|
Cell Recovery (normalized to control)
|
| 1400 |
20 |
1 |
35.08% |
30.67%
|
| 1200 |
20 |
2 |
31.05% |
26.99%
|
| 1200 |
30 |
1 |
29.48% |
30.67%
|
Will use these values to create a list of transfection conditions for 12-well plates tomorrow.
| 
PcTF breast cancer
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