User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2009/05/18

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The Daily Grind

  • Got GFPuv expressing 1B12, 1G12, 2B2, LCh2, LTu1, LDi6
  • did pDSK-GFPuv miniprep
  • made overnights of about 20 more endophytes in R2A for electroporation
  • tested BACs 1A1, 1N1, 1O1, 1O2, and 1P1 in TSB with double strength chloramphenicol, and with BAC induction : looking for transgenic smell I sensed in 1O1 before...

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