User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2009/07/07

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The Daily Grind

  • Gonna do a growth promotion assay on Arabidopsis Col-0 testing Kp342, Gluconacetobacter azotocaptans DS1, E. coli DH5alpha, Bacilus GB03 (from Pare), and seed endophytes 1A4, 1B4, 1B6, 1B7, 1F6, 1G5, 1G6, 2A10, 2B2, 2B6, 2D11. These will be grown overnight and used to coat surface sterilized Col-0 seed with endophytic suspensions in sodium phosphate buffer with an OD of 0.1 (in E. coli 1.0 OD600 = approx. 2.5 x 10^8 cells/ml). H2O will be used as a negative control, and innoculated seeds will be left to dry in the laminar flow cabinet. Dry seeds will be placed on halfstrength Murashige and Skoog (1/2 MS) salt medium with 0.8% Bacto-agar as the gelling agent. After overnight stratification at 4 degrees, plants will be transferred to a light box with a 9-h light cycle, 2,000 lux, with the temperature of 20 ± 4°C.
  • My arabidopsis (Col-0, salk, sail, pom1, and popctl1 overexpressors) have now been growing in short day conditions for 60 days and are quite big. They have recieved no extra nutrients in the soil, and will be now exposed to long day conditions and wind from a small fan on the low setting. I watered them today and depending on how they look tommorow, I will start the above treatment to induce bolting.



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