User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2009/08/31

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The Daily Grind

  • Recieved two ACC deaminases with promoters in E. coli from Dr. Glick.

500: E. coli DH5alpha with plasmid pRKTACC (this is tet resistant plasmid pRK415 with ACC D from Enterobacter cloacae UW4 under control of a tet resistance promoter)

501: E. coli DH5alpha with plasmid pRKlACC (this is tet resistant plasmid pRK415 with ACC D [plus its native RBS] from Enterobacter cloacae UW4 under control of a LAC promoter)

  • also did lots of cloning (the Xba/Spe cut PDS into the second RNAi site in the Maize intron construct)
  • tommmorow will make glycerol stocks of all endophytes and make plates for biochemical screens.


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