User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2009/09/01

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The Daily Grind

  • There's a problem with acetoin production in E. coli at the levels the PSBA promoter at high copy number induces: no cells survive. Therefore I better try this using an inducible promoter like the arabinose one.
  • Screening alsD in pSB1A2 colonies for positives using PCR
  • Sending some samples for sequencing including Norma's BRP
  • Consolidating all the clean colonies for plate 3 glycerol stock and for slow growing plate 4
  • Making plates for biochemical screen and stamping them at the end of the day before freezing the glycerols

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