User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2010/06/03

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The Daily Grind

  • To do: plant pioneer seedlings in greenhouse for injection assay (Arabad::GFP working in planta? + can an assortment of pDSK-GFPuv tagged endophytes make it to the root?)
  • To do: gel extract Nde/Spe cut plasmids and do ligation of ACC to Arabad/Terminator in pDRIVE.
  • To do: Run bigdye sequencing of remaining PCR products of soil swap endophytes.
  • To do: plan for seed coating experiment (sodium alginate + some isolates)

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