User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2011/11/29

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Remains of the Day

  • Arrange with Steve Evans for more liquid nitrogen (maybe get to extract RNA today if you do)
  • Do nested PCR on NSA3/NCL2 primer PCRs from yesterday, using ITS1F and ITS4 (doing 35 cycles of gradient with 1ul of PCR product as template)
  • Do 799F and 1392R PCR on SantaMaria soil samples (spec them for concentration first)


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