User:David Johnston Monje/Notebook/Maize Endophyte Biofertilizers/2013/05/28

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Remains of the Day

  • Gonna work with Sole to do PCRs on Tilbury untreated, pasteurized and autoclaved rhizospheres, and roots from untreated (from rep 1) to clone and sequence and expand identification. Also gonna do Tilbury untreated rhizosphere from rep 2. Doing all 5 samples, then pooling them (but leaving some out if they look homogenous). Ligated them overnight at 4 degrees (mixed 80 of PCRs per treatment and eluted in 25 ul of TE then used 5 ul of this per ligation with 6 ul of ligation buffer).

image: May28,2013Gel.tif



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