User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/07/10

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Immunocytology T1

Standard Procedure: Immunocytology

Antibodies Used:

Antibody Secondary/Primary Fluorophore Dilution Catalog
Glucagon (FL-180) Primary --- 1:500 sc-13091
Insulin Primary --- 1:250 ab7842
Goat Anti-Guinea Pig IgG Secondary Red-Alexaflour 594 1:1000 ab1501888
Rabbit Anti-Glucagon Secondary Green 1:1000 ---
Hoechst 33325 Secondary Blue 1:1000 ---

Results

  • Too few cells fixated for useful results. Plates were at near 0% confluence.
  • DAPI shows faint blue coloration in some cells, however no insulin or glucagon detected.



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