User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2014/11/18

Cell Fate Switch by Synthetic Transcription Factors

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Pancreatic Cell Culture Insert content here... RT-PCR Protocol Note on bench to make 750nM primers ELISA Data Analysis= Experiment showed no concentration of Insulin or Glucagon (low absorbance) Control experiment (only substrate) turned very blue (high absorbance)            Added TBST in last washing step - activation of substrate? Bioinformatics GO Term Analysis Should go over GO Terms again G-protein linked to secretion, acceptance of glucagon/insulin (importance) What GO Terms should we see?            GSIS, Secretion, Packaging, Membrane Permeation Integral Proteins...