User:David K. Barclay/Notebook/Controlling Pancreas Cell Fate Using Transcription Factors/2015/02/26

Cell Fate Switch by Synthetic Transcription Factors

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RT-PCR Testing primers DB07-DB13 against cDNA06 Followed Sybr Green RT-PCR Protocol 7 Primers (primer and template master mixes different) Pancreas Cell Passage by cell split protocol Cells at 70% confluency in T-25 starter medium Deactivate 2mL of trypsin with 4mL of DMEM/20% FBS (6mL cells) Transfer 3mL of cells into 7mL of DMEM/20% FBS (2 T75 flasks) Two T75 flasks of TC1-alpha Pancreatic cells in 37 incubator