User:Dhea Patel/Notebook/Hemoglobin Project/2014/02/24

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OPD in H2O at 15C

  1. Stock solutions:
    1. OPD
      1. 3.9 mg OPD in 3.9mL H2O
    2. H2O2
      1. 0.114mL 30% H2O2 in 0.887mL H2O
  2. Experimental Run
    1. Sample chamber set to 5C and to stir
    2. 2.025mL OPD stock solution
    3. 0.75mL H2O2 stock solution
    4. Start Data Collection
      1. 1ms Integration
      2. 10 scan average
      3. Wait 1 minute after each scan
      4. Files saved as: Mb_OPD_H2O2_Phosphate_MeOH_5_SCANNUMBER.txt
    5. 0.225mL H2O added just before the third scan
      1. This is denoted in the data file for the third scan



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