User:Dhea Patel/Notebook/Phosphorylation/2012/07/12

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Contents

Objective

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Description

  1. Solubility tests were run on RuATP.
  2. After determining the solvents that dissolved the RuATP, 6 crystal growth systems were set up, using 10mg of RuATP per system. The RuATP was placed in a clean, small vial and filled with just enough solvent to dissolve all the RuATP. The small vial, uncovered, was placed in a clean, larger vial and filled with just enough precipitant so that the small vial did not float in the larger vial. The system was placed in an area where it would not be disturbed and covered securely with the large vial's lid.
         Solvent          Precipitant
         H2O              Acetone
         H2O              Chloroform
         Methanol         Acetone
         Methanol         Chloroform
         Ethanol          Acetone
         Ethanol          Chloroform

Data

RuATP was soluble in:

  • 0.1M TEAB
  • Methanol
  • Ethanol
  • Pyridine
  • Water
  • Iso-butyric Acid

RuATP was partially soluble in:

  • Triethanolamine
  • Isopropanol
  • N,N-Diethylformamide

RuATP was not solube in:

  • Dichloromethane
  • Chloroform
  • Acetone
  • 2-Butanone
  • Ethyl Acetate
  • Tetrahydrofuran
  • Acetonitrile
  • Triethylamine

Notes

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