User:Dileep D. Monie/Notebook/BBF Standards Measurement/2008/04/01

From OpenWetWare

Jump to: navigation, search
BBF Standards: Promoter Measurement Test Main project page
Previous entry      Next entry


  • Added 5ml of Supplemented M9 Media containing 20 μg/ml Kanamycin (Sigma K0254; lot no. 027K2378) to fifteen 17mm test tubes
  • Added 5ml of Supplemented M9 Media with no antibiotic (for the TOP10 negative control cells) to three 17mm test tubes
  • Inoculated media by picking single colonies from plates streaked on 2008-03-24 (each strain was inoculated in triplicate from different colonies)
  • Grew the cultures at 37°C with shaking at 70 RPM

Personal tools