2008-04-03
- Analyzed flow cytometry data collected on 2008-04-02 using BD CellQuest Pro v5.2
- Forward vs side scatter plot showing TOP10 cells (1% of events are shown) and R1 gate used for statistics
- Histogram overlay showing FL1 signals (GFP) of representative samples from each group (gated on R1) and markers (M1 and M2) used for statistics
- Purple solid: TOP10
- Green line: I20259
- Pink line: I20269
- Light blue line: I20270
- Orange line: I20268
- Dark blue line: I20260
- Calculated geometric means of fluorescence intensities (Isample,MX) for triplicate samples (bounded by appropriate marker)
| TOP10 (M1) | I20259 (M1) | I20269 (M2) | I20270 (M2) | I20268 (M2) | I20260 (M2)
|
|---|
| 1.62 | 1.66 | 34.62 | 78.93 | 133.87 | 170.4
| | 1.65 | 1.68 | 33.44 | 77.06 | 133.29 | 168.55
| | 1.65 | 1.67 | 34.15 | 77.18 | 137.02 | 171.03
|
- Calculated fluorescence intensities in consensus BioBrick™ GFP units (I/II20260) using (<Isample,MX>-<ITOP10,M1>)/(<II20260,M2>-<ITOP10,M1>)
| TOP10 | I20259 | I20269 | I20270 | I20268 | I20260
|
|---|
| 0 | 0.000178197 | 0.192630579 | 0.451926504 | 0.790519938 | 1
|
|
BBF Standards: Promoter Measurement Test
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