AuNP Synthesis
- HAuCl4 Stock Solution
- 42.97 mg HAuCl4 using parafilmed spatula
- 50 mL water in vol flask
- 2.53 mM stock soln
- Volumes Needed For Colloid & Fiber Solutions
|
Lys Colloid |
Lys Fiber |
BSA Colloid |
BSA fiber
|
Au:protein |
< 35 |
> 35 |
< 135 |
> 135
|
[protein stock] |
70.96 μM |
|
17.73 μM |
|
Vol HCl |
988 μL |
988 μL |
988 μL |
988 μL
|
Vol protein |
1.174 mL |
0.881 mL |
1.083 mL |
1.066 mL
|
Au:protein |
30 |
40 |
130 |
140
|
- AuNP Preparations
- 30:1 Au-Lys --> 988 μL Au + 2*587 μL protein + 7.838 mL H2O
- 40:1 Au-Lys --> 988 μL Au + 2*440 μL protein + 8.131 mL H2O
- 140:1 Au-BSA --> 988 μL Au + 2*503 μL protein + 8.006 mL H2O
- Heated in foil covered glass test tubes 80 °C for 3 hr
- Added 24.32 mg CaCl2 ("anhydrous" label, but visibly wet) to 30:1 Au-Lys solution
- Fibers in other two, as expected
- Invertase not dissolved, so not used
|