User:Douglas M. Fox/Notebook/AU CHEM-571 F2011 Lab Support/2014/10/07

Project name

Main project page Previous entry      Next entry

Primary Experiment Over the next 6 weeks, we will continue the dialysis experiments. You will examine the following systems: 0.6 g/L Lysozyme vs CaCl2 using MWCO 3500 0.6 g/L Lysozyme vs HCl using MWCO 20000 30:1 colloid solution vs CaCl2 using MWCO 3500 30:1 colloid solution vs HCl using MWCO 20000 0.6 g/L Lysozyme vs KI using MWCO 3500 30:1 colloid solution vs KI using MWCO 3500 Measurements will include: UV/VIS Bradford analysis (PS cuvettes) for all protein containing solutions pH for all H+ containing solutions Ca2+ ISE for all Ca2+ containing solutions UV/VIS (glass microcuvette) for all protein solutions Fluorescence (glass microcuvette & 100 fold diluted) for all protein solutions Precipitation titration and/or fluorescence and/or electrochemistry for I- containing solutions Consider the following as you decide which concentrations to test Protein concentration in 30:1 colloid solution is about 8 μM Prior class observed CaCl2 induced precipitation at 100 mM Other scientists have found NaCl induced precipitation at 1.5 M We want to determine if colloid precipitate occurs at same conditions as lysozyme precipitation Lower concentrations are typically less precise Additional Tasks During Waiting Periods I- concentration using precipitation titration I- concentration using potentiometer (Ag/AgI electrode) Rerun gel electrophoresis Test protein specific GPC column Convert earlier GPC plots into Excel files Lysozyme kinetics experiment We will start with the I- concentration by titration today