User:Eamonn B. Mallon/Notebook/Monoparental expression in worker reproduction genes/2010/06/14

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Monoparental expression in worker reproduction genes Basic protocol for production of reproducing workers. Mark callows from colony A. Put back in nest for 2 days. Once 2 days old add one worker from colony A plus two callows from B plus some young cocoons. Leave for 7 days. Older worker can assumed to be egg laying. Verify with pictures taken on addition and at 7 days (check that the brood pile has been added to. Store all bees at -80oC Everyday observation for competition point 5 minutes scanning looking for (1) Worker oviposition (2) oophagy (3) egg cell detruction (4) two or more opened egg cells over 2 or more successive days.